Could Cratylia argentea replace Tifton 85 hay on growing and finishing lamb diets in tropical areas?

Legumes shrubs such as Cratylia argentea have an ability to thrive in environments with low water availability and poor soil. On the other hand, forage grasses such as Tifton 85 have a greater demand for inputs to be productive. The objective of this study was to evaluate the performance of growing and finishing Lacaune lambs fed Cratylia argentea hay as an alternative to Tifton 85 (Cynodon spp). Twenty-four Lacaune lambs aged between five and six months (average body weight [BW] 21.50 ± 3.38 kg) were arranged in a split-plot randomized block design. The plots consisted of different Cratylia to Tifton 85 hay proportions (0, 20%, 40%, or 100%, dry matter [DM] basis) as a roughage replacement in the total diet. The subplots represented two evaluation times, entitled “initial period” and “final period”, which consisted of the early seven days of total feces and urine collection, and the last seven days of the experiment, respectively. The lambs were blocked by weight with six replicates per treatment. The results show that the level of Tifton 85 replacement for Cratylia hay in the roughage portion of the lamb diet did not influence (P > 0.05) weight gain (WG), dry matter intake or dry matter digestibility; feed conversion, feed efficiency; and the evaluated nitrogen balance variables. The digestibility coefficient of neutral detergent fiber decreased linearly as Tifton 85 replacement for Cratylia level was increased, which probably happened due to the presence of highly lignified material within the Cratylia hay. However, the alternative legume maintained animal performance of Tifton 85. In conclusion, Cratylia hay can be recommended as a potential substitute for Tifton 85 hay, which requires greater inputs for the production. Cratylia may be considered a feeding strategy for livestock production, especially for smallholder livestock systems and regions with unfavorable soil and climate.

This statement is required for submission and will appear in the published article if the submission is accepted.Please make sure it is accurate.

Unfunded studies
Enter: The author(s) received no specific funding for this work.• NO -Include this sentence at the end of your statement: The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

* typeset
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing Interests
Use the instructions below to enter a competing interest statement for this submission.On behalf of all authors, disclose any competing interests that could be perceived to bias this work-acknowledging all financial support and any other relevant financial or nonfinancial competing interests.This statement is required for submission and will appear in the published article if the submission is accepted.Please make sure it is accurate and that any funding sources listed in your Funding Information later in the submission form are also The authors have declared that no competing interests exist.General guidance is provided below.
Consult the submission guidelines for detailed instructions.Make sure that all information entered here is included in the Methods section of the manuscript.
Ethics approval: all experimental procedures were approved by the Ethics Committee on Animal Use (CEUA) at the Federal University of Minas Gerais (approval no. 192/2020).and "final period", which consisted of the early seven days of total feces and urine collection, and the last seven days of the experiment, respectively.The lambs were blocked by weight with six replicates per treatment.The results show that the level of Tifton 85 replacement for Cratylia hay in the roughage portion of the lamb diet did not influence (P > 0.05) weight gain (WG), dry matter intake or dry matter digestibility; feed conversion, feed efficiency; and the evaluated nitrogen balance variables.The digestibility coefficient of neutral detergent fiber decreased linearly as Tifton 85 replacement for Cratylia level was increased, which probably happened due to the presence of highly lignified material within the Cratylia hay.However, the alternative legume maintained animal performance of Tifton 85.In conclusion, Cratylia hay can be recommended as a potential substitute for Tifton 85 hay, which requires greater inputs for the

Introduction
Tifton 85 (Cynodon spp.) is one of the major grasses used for hay production in Brazil (Fonseca and Martuscello, 2010).Nonetheless, tropical grasses such as Tifton 85 can have its feed value considerably reduced due to lower digestibility and quality over water deficit periods.Moreover, its yield and use are linked to the adoption of management practices associated with the intensification of inputs use such as chemical fertilization (Jaramillo et al., 2018) and complementary irrigation.
The increased use of chemical fertilizers is linked to greater greenhouse gas (GHG) emissions and depletion of water resources, which happens due to nitrous oxide emissions and runoff, respectively (Snyder et al., 2009), thus, decreasing the sustainability of livestock systems.In the same context, a rise in nitrogen fertilizer costs have led nitrogen (N) fixing crops, such as legume shrubs, to become more attractive for producers, particularly of smallholder livestock systems.
Several leguminous shrubs and trees have been identified as feed alternatives for ruminants in tropical areas (Castro-Montoya and Dickhoefer, 2020;Wanapa et al., 2021).However, its use is yet to be adopted, possibly due to a lack of consensus on the most suitable species and how to incorporate them into tropical production systems (Ates et al., 2018;Castro-Montoya and Dickhoefer, 2020).
Cratylia argentea (Cratylia) is a perennial legume shrub with a feeding potential for livestock in tropical areas.The species is native to the Brazilian Savanna (Cerrado) but is also found in other biomes such as the Amazonian Rainforest and Caatinga (Queiroz, 1991;Queiroz and Coradin, 1996).Although its Brazilian origin, Cratylia have previously been planted for livestock feeding on other countries such as Mexico (Valles-de la Mora et al., 2017), Costa Rica (Ibrahim et al., 2001) andColombia (Hess et al., 2004).
Cratylia is very competitive not only in terms of mass production but largely because of its ability to symbiotically associate with nitrogen-fixing bacteria as well.It has great crude protein (CP) concentration and yielding potential, a symbiotic ability to fix atmospheric N (Matrangolo et al., 2018).Cratylia can maintain green leaves in critical periods of water deficit, thanks to its deep and spread root system (Matrangolo et al., 2018).In their initial growth phase, the Cratylia rootlets develop an external tissue similar to a cork, which helps to minimize water loss (Matrangolo et al., 2018).Those features allow Cratylia to keep growing during the dry season, despite being grazed or harvested (Argel and Lascano, 1998).The same authors reported that 30-40% of Cratylia's yield may occur in the dry season, which makes a great feedstock to fill forage gaps in tropical regions.Another important characteristic of this legume is its tolerance to acidic soils with high aluminum saturation and low natural fertility (Maas, 1995;Luz et al., 2015).Dry biomass accumulation by this legume ranges from 14 to 21 t ha -1 yr -1 , without fertilizer application or supplemental water, as reported in previous research (Pizarro et al., 1996).
Several studies have demonstrated the benefits of including legume shrubs as an alternative for diet and performance improvement on ruminants (Hess et al., 2004;Valles-de la Mora et al., 2017;Herrera et al., 2021).Those studies found that these plants constitute a path towards sustainability, whether through direct grazing or for the enrichment of diets supplied in a trough (Ates et al., 2018;Castro-Montoya and Dickhoefer, 2020 Cratylia was associated with sprayed molasses.In addition, there was a 43% increase on intake of Cratylia, when treated with molasses, which also resulted in a greater intake (>13%; P < 0.05) of H. rufa.
With that being said, it is assumed that Cratylia hay might be an alternative to hay of forage grasses in the diet of finishing lambs.The objective of this study was to evaluate the performance of finishing lambs fed diets with increasing levels of Cratylia hay to replace Tifton 85 hay as the roughage source.

Study area
The where Cratylia was planted for hay is described in Table 1.The Craltylia hay was produced entirely without the use of chemical fertilizers based on the potentially autogamous characteristic of the legume and to maintain the low production cost (Andersson et al., 2006).Tifton 85 hay was purchased from a local dealer and presented a dry matter (DM) of 91.25%, CP 13.2% and NDF 68.98%, which was used to formulate the feed mixture.The agronomical accessions for the harvested Cratylia hay are presented in Table 2.

-----------------(mg kg -1 ) ------------------(dag kg -1 )
28/03/13 5.5 18 2.18 39.26 3.44 Soil pH was measured using a 1:1 soil:water, NO3-N was measured using a 2M KCl extract, and plant available P and K were extracted using a Mehlich-3 solution (Mehlich, 1984).To produce enough forage mass for the totality of animals, after each harvest, the produced hay was stored in raffia bags and packed in drums to avoid contamination, until the beginning of the feeding treatments at LAMA/LACA, which started in November 2020 and lasted until January 2020.All harvested material was cut through a forage chopper then spread in a 10-cm layer on a cemented area for drying in the sun and turned frequently for drying to the point of hay, which happened when the Cratylia reached a DM content of 85%.The interval of exposure to the sun for the material to reach the point of hay varied between 48 and 72 h, depending on the weather conditions.During the night, or in case of rain, the material was covered with canvas to prevent moisture.
The Tifton 85 and Cratylia hay were ground to a particle size of 5 mm, homogenized, and then mixed with the other ingredients of the experimental diets according to the replacement levels (Table 4), as described by Lima (2016).

Feeding trial
Twenty-four Lacaune lambs aged between five and six months (21.5 ± 3.38 kg) were divided into groups according to their initial weight (Table 5).The lambs were housed in individual metabolic cages (1.5 × 0.7 m) equipped with a feeding and water trough, and a system for separate collection of feces and urine, inside a covered masonry shed with an exhaust system at LAMA/LACA laboratories.The total experimental period was 64 days, the first 21 of which were used as a period of adaptation to the experimental diets and cage management, followed by 43 days of intake and weight gain (WG) measurements.Digestibility trials were conducted in two periods, here entitled "initial" and "final".The initial period consisted of 7 days of total collection of feces and urine, which began after the adaptation period (5 to 6 months, 26.8 ± 3.26 kg), and the final period began in the last 7 days of the experiment (6 to 7 months, 37.5 ± 3.26 kg).
Feed was supplied twice daily (08h00 and 14h00) for ad libitum intake (200 g/kg orts, as-fed basis).Supplied feed and orts were weighed daily on an electronic scale.Each of the diets was composed of different levels of Cratylia hay to replace Tifton 85 hay (Table 4).The diets were formulated to meet the requirements for finishing lambs with a DM intake of 4% BW and an average daily gain (ADG) of 200 g, thus, a 16% CP and total digestible nutrients (TDN) of 75%, following the NRC (2007).All diets were supplied as a total mixture ratio and adjusted daily allowing up to 20% orts.
Live weight was measured weekly from the beginning of the experimental period, in the morning period before the feed was supplied, using a platform scale.Initial live weight (ILW) was determined immediately after the end of the adaptation period, and final live weight (FLW) at 43 days of the experiment.The ADG was determined by the following equation: ADG = ((FLW-ILW)/(Number of days in the feedlot).Dry matter intake (DMI) was individually calculated as the difference between the amount of feed supplied and daily orts.
The digestibility trial involved total collection of feces and urine on two occasions: (1) in the first week and (2) in the last week of the experimental period.
Samples of supplied feed, orts, and feces were analyzed for the dry matter (DM), ash, and nitrogen (N) contents according to AOAC (1990) and the neutral detergent fiber (NDF) content according to Van Soest et al. (1991).The total tract apparent digestibility coefficient (ADC) was determined as follows: ADC = ([OF -Intake -CF]/OF -Intake) × 100, which was proposed by Maynard et al. (1984), The nitrogen content of the sample was determined by the micro Kjeldahl method (Campos et al., 2004).Nitrogen balance was calculated according to Gentil et al. (2011).

Statistical analyses
The 24 lambs were allocated in a randomized block design with four blocks, where each block was randomly assigned with a dietary treatment of 0, For performance variables, the fits of polynomial regression models were tested to obtain the equation as a function of the Tifton 85 replacement levels for Cratylia hay or collection periods.
Analyses were carried out using R statistical analysis software (Core Team, 2021).For all analyses, the statistical assumptions of normality and homoscedasticity were evaluated by the Shapiro-Wilk and Bartlett tests, respectively.An effect was considered significant when the α error was less than 5%.

Intake and digestibility of Cratylia hay
Dry matter intake did not differ (P > 0.05) in response to Tifton 85 replacement levels for Cratylia (Table 6).However, there was an increase of 12.5%, 17.64%, 18.75% and 16.67% in DMI with the respective increase in the proportions (0%, 20%, 30%, 40% and 100%) of Cratylia in the final collection period (P < 0.05), compared with the initial collection period.As far as the digestibility coefficient of DM, no difference (P > 0.05) was observed with Tifton 85 replacement levels for Cratylia hay or collection period.
The digestibility coefficients of OM showed a significant (P < 0.05) decrease from 715.1 to 681.7 g/kg DM, with increased proportions of Cratylia in the roughage portion of the diet to replace Tifton 85.The same trend was observed on CP, wherein greater proportions of Cratylia decreased (P < 0.05) the CP digestibility from 743.8 to 710.9 g/kg DM, from control to the treatment with 100% of Cratylia hay.Regarding NDF, there was also a steady decrease (P < 0.05) in digestibility, which ranged from 606 to 404.3 g/kg DM, with increased levels of Cratylia.
In respect to collection periods, the digestibility coefficient of OM had increased (P < 0.05) from 712.2 to 727.2, 701.6 to 715.6, and 681.7 to 700.1 g/kg DM at 20%, 40%, and 100% from initial to the final collection period, respectively.
The same was observed for NDF, which the digestibility increased (P < 0.05) from 571.3 to 609.1, 512.9 to 538.8, 404.3 to 454.0 g/kg DM at 20%, 40%, and 100% from initial to the final collection period, respectively.Nevertheless, no significant differences between collection periods were observed on digestibility coefficients of DM and CP.
The feed conversion (FC) and feed efficiency (FE) did not differ (P > 0.05) with Tifton 85 replacement levels for Cratylia's hay.However, FC was greater in the final period (P < 0.05), while FE was greater in the initial period.Dry matter intake was positively (P<0,01) influenced by weeks of hay feeding, whereas DMI was increased by 0.858 g per animal per week (Fig. 1A).
As far as WG, a similar pattern was observed, considering that WG increased 1.93 kg per animal per week (P<0,01) (Fig. 1B).The digestibility coefficient of NDF decreased linearly by 0.18% every week with the Tifton 85 replacement for Cratylia hay (% DM) in the diet (Fig. 2).
Therefore, as estimated by the regression equation, the replacement levels of 20, 40, and 100% would cause a decrease on NDF digestibility by 57.42, 53.76, and 42.77%, respectively.Although, this behavior did not negatively influence lamb performance, an accentuated decrease in NDF digestibility was observed at 100% replacement of Tifton 85 with the Cratylia.

Effects of Cratylia hay on nitrogen variables
The level of Tifton 85 replacement for Cratylia in the roughage did not affect any of the nitrogen balance variables (P > 0.05) (Table 7).As far as the collection period, the N intake, fecal N, absorbed N, and urinary N were greater (P < 0.05) in the final collection period.On the other hand, the proportion (%) of Retained to Absorbed N and Retained N to N Intake was greater (P < 0.05) in the initial period.In regard to Retained N (g/day), no changes (P > 0.05) were observed according to collection periods.

Lambs' performance on Cratylia argentea hay
In most of the measured parameters, the evaluated lambs performed similarly under diets with Tifton 85 replacement for Cratylia hay.Therefore, revealing the capability of Cratylia to be used as animal feed.The use of alternative feedstuff has been a common practice to decrease production cost on livestock systems (Awawdeh, 2011).Producers without the necessary inputs to grow or purchase Tifton 85 hay, may have an alternative with Cratylia, which is a sustainable feedstuff, since it does not require the use of chemical N fertilizers for high yield and quality biomass production (Andersson et al., 2006).
For the variables of DMI, dry matter digestibility (DMD), FC and FE, Cratylia and Tifton 85 performed the same as well.Dry matter intake was not related to differences in digestibility between the two forage species since the dietary replacement of Tifton 85 for Cratylia did not increase DMD.The particle size uniformity of the diet supplied to the lambs did not allow them to select ingredients, which explains the absence of differences in DMI.Thornton and Minson (1973) reported that the voluntary intake of tropical legumes (when not limited by anti-nutritional factors) is generally greater than that of equally digestible grasses, which may be a consequence of a shorter retention time of legumes in the digestive tract.Likewise, feed particle size may have also influenced the response of the DM digestibility coefficient.The effectiveness of fiber was broken by the particle size of the roughage, so the intestinal passage rate could not reveal possible interactions between the roughages used (Tifton 85 or Cratylia hay).The digestibility coefficient values found in this study are similar to those described by other authors (da Trindade Silva et al., 2021), which included shrub hay and tree legume species as roughage sources in the total diet of feedlot lambs.
The greater DMI with weeks of hay feeding present in this study might be related to an increase in energy required for maintenance, compared to earlier periods of lower BW (Brody, 1945). Cabral et al. (2008) have also found increased DMI and WG in animals with increased weight when evaluating confined lambs.The increased WG supports the findings that Cratylia did not negatively affect the performance on evaluated lambs.The addition of Cratylia on animal feeds was previously reported to increase (P < 0.05) feed intake from 6.6 to 7.8 and 8.7 kg DM/day, when creole dairy cows were fed sorghum silage alone and supplementation with 2 and 3 kg DM of Cratylia, respectively (Sanchez and Ledin, 2006).

Digestibility
In the matter of OM, CP and NDF digestibility, the increased levels of Cratylia resulted in lower digestibility.Pinzón and Mariño (2010) also reported lower CP and NDF digestibility coefficients in Cratylia hay compared with wholeplant silage or meal.The greater NDF and acid detergent fiber (ADF) contents and lower digestibility of sun-dried forages are attributed to the negative effects of ultraviolet radiation, which can induce the Maillard reaction and thereby increase fiber-bound protein (Van Soest et al., 1991;Dzowela et al., 1995).As an example, nitrogen contents bound to the plant cell wall, which become unavailable to the rumen microbiota (Harper and McNeil, 2015).Moreover, the plants used for hay production were harvested for high DM production (90 days of regrowth) and cut as a whole, including stems and senescent leaves.
Therefore, there was no selection for the less lignified material, which may have contributed to greater indigestible fiber content in the diet.
The NDF content of the plants at the time of harvest in this study was similar to the 67.54% and 65.68% found by Herrera and Arguedas (2016) in Cratylia plants harvested at 90 days in the rainy and dry seasons, respectively.It is important to highlight that the lignin present in shrub and tree legumes, as in the case of Cratylia, may be less degradable in the rumen compared with the lignin of grasses (Harper and McNeil, 2015).However, this knowledge is derived from reports of studies with temperate forages, so the differential action of lignin in tropical legumes is yet to be validated (Castro-Montoya and Dickhoefer, 2020).

Feed conversion and feed efficiency
The greater FC and lower FE in the final period might be attributed to the fact that lambs on the last seven days before finishing were heavier and had a lower growing factor, which limits FE.Pires et al. (2000) suggest that older lambs in the finishing phase tend to have different nutrient demands and composition of weight gain (e.g., more fat than muscle gain), thus, lower FE.Polli et al. (2019) evaluated finishing lambs in lower and higher heat conditions and found a FE decrease of 12.8% and 20.4% on feedlot lambs as the finishing period progressed, respectively.Bowen et al. (2006) implied that FC might be associated with several factors including feed intake, increased age and liveweight of the animal, which explains the findings of this research.

Nitrogen variables
About the increase in N variables at the final collection period (N intake, Fecal N, Absorbed N, Urinary N), it is assumed that greater N content on Cratylia than Tifton 85 (Table 2) justifies the finding.Hess et al. ( 2004) described greater urinary N excretion in lambs fed diets supplemented with dry leaves of Cratylia, in comparison with the group fed a basal diet with hay of Urochloa dictyoneura cv.Lanero.The authors relate this result to the increased urine volume and intake of available water.A similar behavior was observed in the present experiment, which N excretion was greater in the urine than in the feces, especially in the heavier lambs.The greater proportion (%) of Retained to Absorbed N and Retained N to N Intake in the initial period possibly happened due to a greater demand of the growing lambs for proteins.Since part of the N further is synthesized into proteins by rumen microorganisms (Aquino et al., 2007), to supply the protein demand and increase muscle growth (Prado et al., 2014;Rezaei et al., 2014).

Conclusion
Overall, the replacement of Tifton 85 for Cratylia hay tested demonstrated that lamb performance can be maintained, regardless of replacement level used in the diets.Thus, might be recommended as a food intervention strategy to support livestock production at no cost of chemical fertilizers, since none was used for Cratylia hay production.
The decreased digestibility of NDF, OM and CP on Cratylia's hay may be linked to the method used to preserve the feed and harvest timing.Therefore, further research is expected to identify the parameters that influenced the chemical characteristics of this plant.
the submission guidelines for detailed requirements.View published research articles from PLOS ONE for specific examples.
with the following details: Initials of the authors who received each award • Grant numbers awarded to each author • The full name of each funder • URL of each funder website • Did the sponsors or funders play any role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript?
Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation declared in your Financial Disclosure statement.View published research articles from PLOS ONE for specific examples.NO authors have competing interests Enter: The authors have declared that no competing interests exist.Authors with competing interestsEnter competing interest details beginning with this statement: I have read the journal's policy and the authors of this manuscript have the following competing interests: [insert competing interests here] /A" if the submission does not require an ethics statement.

Format
or tissues) Provide the name of the Institutional Animal Care and Use Committee (IACUC) or other relevant ethics board that reviewed the study protocol, and indicate whether they approved this research or granted a formal waiver of ethical approval • Include an approval number if one was obtained • If the study involved non-human primates, add additional details about animal welfare and steps taken to ameliorate suffering • If anesthesia, euthanasia, or any kind of animal sacrifice is part of the study, include briefly which substances and/or methods were applied • Field Research Include the following details if this study involves the collection of plant, animal, or other materials from a natural setting: to make all data underlying the findings described fully available, without restriction, and from the time of publication.PLOS allows rare exceptions to address legal and ethical concerns.See the PLOS Data Policy and FAQ for detailed information.Yes -all data are fully available without restriction Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation A Data Availability Statement describing where the data can be found is required at submission.Your answers to this question constitute the Data Availability Statement and will be published in the article, if accepted.Important: Stating 'data available on request from the author' is not sufficient.If your data are only available upon request, select 'No' for the first question and explain your exceptional situation in the text box.Do the authors confirm that all data underlying the findings described in their manuscript are fully available without restriction?Describe where the data may be found in full sentences.If you are copying our sample text, replace any instances of XXX with the appropriate details.If the data are held or will be held in a public repository, include URLs, accession numbers or DOIs.If this information will only be available after acceptance, indicate this by ticking the box below.For example: All XXX files are available from the XXX database (accession number(s) XXX, XXX.).• If the data are all contained within the manuscript and/or Supporting Information files, enter the following: All relevant data are within the manuscript and its Supporting Information files.
where AD = apparent digestibility; OF = offered feed [(offered feed amount in kg DM) x (offered nutrient content in % DM)/100]; Intake = orts [(removed orts feed in kg DM) x (orts nutrient content in % DM)/100]; and CF = fecal output [(amount of collected feces in kg DM) x (collected feces content in % DM)/100].Urine was collected in plastic containers containing a hydrochloric acid solution (6N HCl) to keep the urine pH below 2 and prevent the loss of nitrogen by volatilization.The total collected material was quantified, homogenized, and filtered through cotton gauze and a 10% sample was taken and stored at -18 °C.
SE = standard error; L = level of Tifton 85 replacement for C. argentea hay in the roughage to replace Tifton 85 hay; C = collection period; L×C = interaction.

Fig. 1 .
Fig. 1.Body Weight of lambs (A) as a function of weeks of hay feeding and Dry Matter

Financial
Disclosure Statement: The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.This work was supported by the National Council of Technological and Scientific Development (CNPq, Brazil #140314/2019-9), Minas Gerais State Agency for Research and Development (FAPEMIG, PPM-00639-18) and the Coordination for the Improvement of Higher Education Personnel (CAPES, Brazil #88887.667823/2022-00).
Mora et al. (2017)a et al. (2017)evaluated the development of grazing heifers (F1 Holstein x Zebu) on Brachiaria brizantha cv.Toledo alone vs the association of Cratylia-Brachiaria brizantha Ibrahim et al. (2001)6) weight gain on animals grazing the Cratylia treatment.The same study also observed CP levels of over 20% in Cratylia, which were very consistent throughout the experiment.Andersson et al. (2006)evaluated the nutritive value of 38 Cratylia accessions harvested at 8 weeks of regrowth and found a mean CP of 20,6% and 22% and in vitro digestible dry matter (IVDDM) of 64,1% and 64,9% for dry and rainy season, respectively.Ibrahim et al. (2001)evaluated the association of Hyparrhenia rufa pasture and fresh Cratylia argentea as a protein supplement with or without molasses (diluted in the water trough) for dual purpose cattle in the dry season of Costa Rica.The results showed an increase of IVDDM from 55.6 to 60.8% (P < 0.01) when study was conducted at the Brazilian Agricultural Research The unit where Cratylia was planted is in a region with a Cwa climate type according to the Köppen classification system, i.e., savanna climate, with dry winters and humid-rainy summers.Cratylia was transplanted from a growth chamber to a research site on March 28 th 2013, and several trials were conducted in the area until 2019 when the current research began.Cratylia's shrubs were planted in an area of 105 m 2 , spaced at 1 m between rows and a total of 126 plants, or 12,000 plants/ha -1 .The area size was determined based on the number of available Cratylia's sprigs found available with research peers before planting, since commercial seeds were not available at the time.The soil test for the area

hay and feed management
To establish a uniform regrowth, all Cratylia's plots were mowed in January 2019.The first harvest for data collection happened in April of the same year and was repeated every other 90 days, until April 2020 (Table3), leaving a 25 cm stubble height (Pinzón and Mariño, 2010), without the selection of less lignified material.During the haymaking period, temperatures were between 17.74 and 29.24 °C, average rainfall was 885 mm, and relative humidity was 66.56% (NASA/POWER, 2022).

Table 4 .
Percentage and chemical composition of experimental diets.

Table 5 .
Block distribution of lambs.

Table 6 .
Intake and digestibility of dry matter and nutrients, feed conversion, and feed efficiency of feedlot Lacaune lambs fed diets containing different levels of C. argentea hay as a replacement Tifton 85 hay at two evaluation times.

Table 7 .
Nitrogen balance in feedlot Lacaune lambs fed diets containing different levels of C. argentea hay replacing Tifton 85 hay at two evaluation times.